Absence of EpCAM in cervical cancer cells is involved in slug induced epithelial-mesenchymal transition

Abstract Background Slug (Snai2) is a pivotal player in initiating epithelial-mesenchymal transition (EMT) through its trans-suppression effect on E-cadherin various normal and malignant cells. In this study, the positive of promoting cell motility metastasis cervical cancer was further confirmed study. Methods RNA-Seq performed to explore potential molecules that participate Slug-mediated EMT The negative correlation between EpCAM expression cells detected linked them with vitro migration invasion assay, vivo experiments, luciferase reporter assay Chromatin immunoprecipitation. Results Transcriptome sequencing analysis revealed epithelial adhesion molecule (EpCAM) significantly decreased Slug-overexpressing SiHa Simultaneously, an absence observed Further studies binding E-boxes proximal promoter region Restoring by transiently transfecting recombinant plasmid attenuated promoted growth. Moreover, human squamous carcinoma (SCC) samples verified using Pearson analysis. Conclusions These results demonstrated under probably participated Slug-regulated tumor metastasis. Additionally, study supports way for initiate progression addition inhibiting E-cadherin.